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Comparative Study
. 2010 Feb;48(2):612-4.
doi: 10.1128/JCM.01402-09. Epub 2009 Dec 9.

Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid

Berthe-Marie Njanpop Lafourcade  1 , Oumarou Sanou, Mark van der Linden, Natalia Levina, Meryem Karanfil, Seydou Yaro, Tsidi A Tamekloe, Judith E Mueller
Affiliations
Comparative Study

Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid

Berthe-Marie Njanpop Lafourcade et al. J Clin Microbiol. 2010 Feb.

Abstract

We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the serotype information yield from 40% of cases (isolate testing) to 90%.

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References

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