Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2010 Feb;48(2):612-4.
doi: 10.1128/JCM.01402-09. Epub 2009 Dec 9.

Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid

Berthe-Marie Njanpop Lafourcade  1 , Oumarou Sanou, Mark van der Linden, Natalia Levina, Meryem Karanfil, Seydou Yaro, Tsidi A Tamekloe, Judith E Mueller
Affiliations
Comparative Study

Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid

Berthe-Marie Njanpop Lafourcade et al. J Clin Microbiol. 2010 Feb.

Abstract

We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the serotype information yield from 40% of cases (isolate testing) to 90%.

PubMed Disclaimer

References

    1. Adjogble, K. L. S., M. Lourd, B. M. Njanpop-Lafourcade, Y. Traore, A. F. S. Hlomaschi, K. A. Amegatse, K. Agbenoko, O. Sanou, S. Kroman, J. E. Mueller, and B. D. Gessner. 2007. The epidemiology of Neisseria meningitidis meningitis in Togo during 2003-2005. Vaccine 25(Suppl. 1):A47-A52. - PubMed
    1. Brito, D. A., M. Ramirez, and H. de Lencastre. 2003. Serotyping Streptococcus pneumoniae by multiplex PCR. J. Clin. Microbiol. 41:2378-2384. - PMC - PubMed
    1. CDC. 2009. Multiplex PCR for deducing serotypes from pneumococcal isolates/PCR serotype deduction protocols/PCR deduction of pneumococcal serotypes. http://www.cdc.gov/ncidod/biotech/files/pcr-serotyping-isolates-US-May20.... Accessed 18 July 2009.
    1. Dias, C. A., L. M. Teixeira, M. G. Carvalho, and B. Beall. 2007. Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children. J. Med. Microbiol. 56:1185-1188. - PubMed
    1. Global Alliance for Vaccines and Immunization (GAVI Alliance). 2009. GAVI in the news. http://www.gavialliance.org/media_centre/news/index.php. Accessed 18 July 2009.

Publication types

MeSH terms

LinkOut - more resources