Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb;48(2):599-602.
doi: 10.1128/JCM.01828-09. Epub 2009 Dec 9.

Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations

S A Moser  1 , S C Mitchell, J D Ruby, S Momeni, R C Osgood, J Whiddon, N K Childers
Affiliations

Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations

S A Moser et al. J Clin Microbiol. 2010 Feb.

Abstract

Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Dendrograms of rep-PCR (A, C, and E) and corresponding PFGE patterns (B, D, and F) of S. mutans isolates from mother/child pairs. Samples were labeled as follows: mother's plaque, M; child's plaque, BP; a sibling's plaque, SBP; plaque from child's caries, BC. The corresponding rep-PCR and PFGE types are noted. Panels A and B are an example of children without a genotype in common with the mother; for panels C and D, mother and child share a genotype and both the child and mother have one unique genotype; for panels E and F, both mother and child have a single genotype that is not shared.
See this image and copyright information in PMC

References

    1. Alam, S., S. Brailsford, R. Whiley, and D. Beighton. 1999. PCR-based methods for genotyping viridians group streptococci. J. Clin. Microbiol. 37:2772-2776. - PMC - PubMed
    1. Bert, F., C. Branger, and N. Lambert-Zechovsky. 1997. Pulsed-field gel electrophoresis is more discriminating than multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis for typing pyogenic streptococci. Curr. Microbiol. 34:226-229. - PubMed
    1. Davis, M. A., D. D. Hancock, T. E. Besser, and D. R. Call. 2003. Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O517:H7. J. Clin. Microbiol. 41:1843-1849. - PMC - PubMed
    1. Douglass, J., Y. Li, and N. Tinanoff. 2008. Association of mutans streptococci between caregivers and their children. Pediatr. Dent. 30:375-387. - PubMed
    1. Goering, R. 2000. The molecular epidemiology of nonsocomial infection: past, present and future. Rev. Med. Microbiol. 11:145-152.

Publication types

MeSH terms

LinkOut - more resources