Prevention of selenite-induced cataractogenesis by rutin in Wistar rats

Abstract

Purpose: To investigate whether rutin retards selenite-induced cataractogenesis in Wistar rat pups.

Methods: On postpartum day ten, Group I rat pups received an intraperitoneal injection of saline. Group II and III rat pups received a subcutaneous injection of sodium selenite. Group III also received an intraperitoneal injection of rutin once daily on postpartum days 9-14. Both eyes of each pup were examined from day 16 up to postpartum day 30. After sacrifice, extricated pup lenses were analyzed for mean activities of catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, and glutathione reductase. In addition, the mean concentrations of reduced glutathione (GSH) and of malondialdehyde were analyzed in samples of lenses and hemolysate.

Results: There was dense lenticular opacification in all of Group II, minimal opacification in 33.3% of Group III, no opacification in 66.7% of Group III, and no opacification in Group I. Significantly lower mean activities of lenticular antioxidant enzymes were noted in Group II, compared to Group I and III. Significantly lower mean concentrations of GSH and higher mean concentrations of malondialdehyde were noted in samples of hemolysate and lens from Group II, compared to the values in Group I and III.

Conclusion: Rutin prevents experimental selenite-induced cataractogenesis in rat pups, possibly by preventing depletion of antioxidant enzymes and of GSH, and by inhibiting lipid peroxidation.

Figure 1

Slit-lamp appearance of lenses in eyes of 16-day-old Wistar rat pups. A: Lenses in normal (Group I) pup eyes have all remained transparent (grade 0 opacity). B: Lenses in cataract-untreated (Group II) eyes have all become densely opaque (grade +++ opacity). C: Lenses in some cataract-treated (Group III) pup eyes have become mildly opaque (grade + opacity).