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. 1991 Feb 15;274 ( Pt 1)(Pt 1):91-5.
doi: 10.1042/bj2740091.

Gly429 is the major determinant of uncompetitive inhibition of human germ cell alkaline phosphatase by L-leucine

C Hummer  1 , J L Millán
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Gly429 is the major determinant of uncompetitive inhibition of human germ cell alkaline phosphatase by L-leucine

C Hummer et al. Biochem J. .

Abstract

The catalytic activity of human placental alkaline phosphatase (PLAP) and germ cell alkaline phosphatase (GCAP) can be inhibited, through an uncompetitive mechanism, by L-Phe. GCAP is also selectively inhibited by L-Leu. Site-directed mutagenesis of five of the 12 residues which are different in PLAP and GCAP revealed that Gly429 is the primary determinant of GCAP inhibition by L-Leu, and Ser84 and Leu297 play a modulatory role in the inhibition.

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