Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb;17(2):263-6.
doi: 10.1128/CVI.00448-08. Epub 2009 Dec 16.

Accuracy of AccessBio Immunoglobulin M and Total Antibody Rapid Immunochromatographic Assays for the Diagnosis of Acute Scrub Typhus Infection

Stuart D Blacksell  1 , Kemajittra Jenjaroen, Rattanaphone Phetsouvanh, Vanaporn Wuthiekanun, Nicholas P J Day, Paul N Newton, Wei-Mei Ching
Affiliations

Accuracy of AccessBio Immunoglobulin M and Total Antibody Rapid Immunochromatographic Assays for the Diagnosis of Acute Scrub Typhus Infection

Stuart D Blacksell et al. Clin Vaccine Immunol. 2010 Feb.

Abstract

Using archived samples, we assessed the diagnostic capacity of a rapid immunochromatographic test (ICT) for the detection of Orientia tsutsugamushi IgM and total antibodies to aid with the diagnosis of acute scrub typhus infection in febrile patients in Laos. The sensitivity and the specificity of the ICT for the detection of IgM were 96.8% (121/125 samples; 95% confidence interval [CI], 92.1 to 99.1%) and 93.3% (98/105 samples; 95% CI, 86.7 to 97.3%), respectively. For the detection of total antibodies, the sensitivity was 97.6% (122/125 samples; 95% CI, 93.1 to 99.5%), but the specificity was much lower, at 71.4% (75/105 samples; 95% CI, 61.8 to 79.8%).

PubMed Disclaimer

References

    1. Blacksell, S. D., D. Bell, J. Kelley, M. P. Mammen, R. V. Gibbons, R. G. Jarman, D. W. Vaughn, K. Jenjaroen, A. Nisalak, S. Thongpaseuth, M. Vongsouvath, V. Davong, P. Phouminh, R. Phetsouvanh, N. P. Day, and P. N. Newton. 2007. Prospective study to determine accuracy of rapid serological assays for diagnosis of acute dengue virus infection in Laos. Clin. Vaccine Immunol. 14:1458-1464. - PMC - PubMed
    1. Blacksell, S. D., R. Luksameetanasan, T. Kalambaheti, N. Aukkanit, D. H. Paris, R. McGready, F. Nosten, S. J. Peacock, and N. P. Day. 2008. Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand. FEMS Immunol. Med. Microbiol. 52:335-342. - PubMed
    1. Horinouchi, H., K. Murai, A. Okayama, Y. Nagatomo, N. Tachibana, and H. Tsubouchi. 1996. Genotypic identification of Rickettsia tsutsugamushi by restriction fragment length polymorphism analysis of DNA amplified by the polymerase chain reaction. Am. J. Trop. Med. Hyg. 54:647-651. - PubMed
    1. Innis, B. L., A. Nisalak, S. Nimmannitya, S. Kusalerdchariya, V. Chongswasdi, S. Suntayakorn, P. Puttisri, and C. H. Hoke. 1989. An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am. J. Trop. Med. Hyg. 40:418-427. - PubMed
    1. Lanciotti, R. S., C. H. Calisher, D. J. Gubler, G. J. Chang, and A. V. Vorndam. 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J. Clin. Microbiol. 30:545-551. - PMC - PubMed

Publication types

LinkOut - more resources