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. 2009 Dec 2;5(7):727-35.
doi: 10.7150/ijbs.5.727.

Identification of Anoectochilus based on rDNA ITS sequences alignment and SELDI-TOF-MS

Chuan Gao  1 , Fusheng Zhang, Jun Zhang, Shunxing Guo, Hongbo Shao
Affiliations

Identification of Anoectochilus based on rDNA ITS sequences alignment and SELDI-TOF-MS

Chuan Gao et al. Int J Biol Sci. .

Abstract

The internal transcribed spacer (ITS) sequences alignment and proteomic difference of Anoectochilus interspecies have been studied by means of ITS molecular identification and surface enhanced laser desorption ionization time of flight mass spectrography. Results showed that variety certification on Anoectochilus by ITS sequences can not determine species, and there is proteomic difference among Anoectochilus interspecies. Moreover, proteomic finger printings of five Anoectochilus species have been established for identifying species, and genetic relationships of five species within Anoectochilus have been deduced according to proteomic differences among five species.

Keywords: Anoectochilus; ITS; SELDI; proteomic finger printing.

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Conflict of interest statement

Conflict of Interest: The authors have declared that no conflict of interest exists.

Figures

Fig 1
Fig 1
Standard curve
Fig 2
Fig 2
Protein peak shape (a and b).
Fig 2
Fig 2
Protein peak shape (a and b).
Fig 3
Fig 3
Affiliation of five plants in Anoectochilus. The number 1-10 in circles shows the relationship of interspecies from near to far.
See this image and copyright information in PMC

References

    1. Editorial Committee of Flora of China, Chinese Academy of Sciences. Flora Reipublicae Popularis Sinicae. Beijing: Science Press; 2000. p. 204.
    1. Institute of Research on Traditional Chinese Medicine. Record of Fujian Materia Medica. Fuzhou, Fujian: Fujian Science and Technology Press; 1982. p. 215.
    1. Zou YP, Ge S, Molecular markers of systematic and evolutionary botan. Beijing: Science Press; 2001.
    1. Campbell CS, Baldwin BG, Donoghue MJ. et al. Phylogeny of the genera of Maloidae (Rosaceae): evidence from sequences of the internal transcribed spacers of nuclear ribosomal DNA sequences and congruence with morphology. American Journal of Botany. 1995;82:903–918.
    1. Kollipara KP, Singh RJ. et al. Phylogenetic and genomic relationships in the genus Glycine Willd.Based on sequences from the ITS region of nuclear rDNA. Genome. 1997;40(1):57–68. - PubMed

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