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. 2010 Mar:59 Suppl 2:S185-6.
doi: 10.1007/s00011-009-0123-6.

Deficiencies in elements involved in TLR4-receptor signalling in RBL-2H3 cells

E Passante  1 , N Frankish
Affiliations

Deficiencies in elements involved in TLR4-receptor signalling in RBL-2H3 cells

E Passante et al. Inflamm Res. 2010 Mar.

Abstract

Objective: RBL-2H3 cells express Toll-like receptors, including TLR4. This study aims to assess various aspects of the TLR4 pathway.

Methods: RBL-2H3 cells were indirectly stained for cell surface TLR4, 25 CD14 and intracellular MyD88 proteins and analysed through flow cytometry for single-colour staining.

Results: While TLR4-receptors are expressed in RBL-2H3 cells, associated elements involved in the signaling pathway, CD14 and MyD88, are not.

Conclusion: Care should be taken if RBL-2H3 cells are used to study aspects of the innate immune system in mast cells.

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