An analysis of amplified insulin gene products in diabetics of Indian origin
- PMID: 2002494
- PMCID: PMC1016776
- DOI: 10.1136/jmg.28.2.97
An analysis of amplified insulin gene products in diabetics of Indian origin
Abstract
We have previously described an increased incidence of the class 3 allele of the hypervariable region (HVR) 5' to the insulin gene in south Indian non-insulin dependent diabetics; this association is absent in Punjabi Sikhs with this disorder. Using the polymerase chain reaction we have amplified parts of the insulin gene from 130 subjects to look for mutations which may be in linkage disequilibrium with the class 3 allele and hence explain its association with non-insulin dependent diabetes (NIDDM). In 23 south Indian subjects with NIDDM, using the restriction enzyme MboII, a B chain mutant (insulin Chicago) was excluded. Two patterns (alpha and beta) were found, representing a PstI polymorphism in the 3' untranslated region of the insulin gene. In subjects homozygous for the class 1 allele, the allelic frequency for alpha was 0.94 (143/152) and for beta was 0.06, in heterozygotes (1,3) alpha 0.63 (54/86) and beta 0.37, and in homozygotes for the class 3 allele alpha 0.18 (4/22) and beta 0.82 (p less than 0.001), thus establishing linkage disequilibrium between these two loci. No differences in allelic frequency were found in the south Indians or Punjabi Sikhs between controls and the different types of non-insulin requiring diabetes (NIDDM, fibrocalculous pancreatic diabetes and maturity onset diabetes of the young) when both groups were matched for insulin genotypes. Thus, although this polymorphism in the 3' untranslated region of the insulin gene is in linkage disequilibrium with the class 3 allele, it does not appear to be any better at predicting diabetes than the class 3 allele itself.
Similar articles
-
Positive association in the absence of linkage suggests a minor role for the glucokinase gene in the pathogenesis of type 2 (non-insulin-dependent) diabetes mellitus amongst south Indians.Diabetologia. 1993 Jul;36(7):633-41. doi: 10.1007/BF00404073. Diabetologia. 1993. PMID: 8359581
-
Polymorphic locus in the 5'-flanking region of human insulin gene and non-insulin-dependent diabetes mellitus.J Formos Med Assoc. 1990 Nov;89(11):965-8. J Formos Med Assoc. 1990. PMID: 1982127
-
Non-insulin-dependent diabetes mellitus and the 5' hypervariable region of the insulin gene in two South African Indian families.S Afr Med J. 1987 Feb 7;71(3):174-8. S Afr Med J. 1987. PMID: 2880401
-
The molecular genetics of diabetes mellitus.Ciba Found Symp. 1987;130:167-83. doi: 10.1002/9780470513507.ch10. Ciba Found Symp. 1987. PMID: 2894928 Review.
-
The genetics of non-insulin-dependent diabetes mellitus in south India: an overview.Ann Med. 1992 Dec;24(6):491-7. doi: 10.3109/07853899209167001. Ann Med. 1992. PMID: 1485944 Review.
Cited by
-
Prevalence of beta allele of the insulin gene in type II diabetes mellitus.Hum Genet. 1994 Mar;93(3):325-8. doi: 10.1007/BF00212031. Hum Genet. 1994. PMID: 8125485
-
In Finland insulin gene region encoded susceptibility to IDDM exerts maximum effect when there is low HLA-DR associated risk. DiMe (Childhood Diabetes in Finland) Study Group.Diabetologia. 1995 Oct;38(10):1223-9. doi: 10.1007/BF00422373. Diabetologia. 1995. PMID: 8690176
-
Genetic variation of the transcription factor GATA3, not STAT4, is associated with the risk of type 2 diabetes in the Bangladeshi population.PLoS One. 2018 Jul 25;13(7):e0198507. doi: 10.1371/journal.pone.0198507. eCollection 2018. PLoS One. 2018. PMID: 30044774 Free PMC article.
-
Allelic divergence in the human insulin gene provides evidence for intragenic recombination events in the non-coding regions: evidence for existence of new alleles.Mol Gen Genet. 1994 Oct 28;245(2):146-51. doi: 10.1007/BF00283261. Mol Gen Genet. 1994. PMID: 7816021
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical