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. 2009 Dec 23:10:626.
doi: 10.1186/1471-2164-10-626.

Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici

Chuntao Yin  1 , Xianming Chen, Xiaojie Wang, Qingmei Han, Zhensheng Kang, Scot H Hulbert
Affiliations

Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici

Chuntao Yin et al. BMC Genomics. .

Abstract

Background: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. In spite of its agricultural importance, the genomics and genetics of the pathogen are poorly characterized. Pst transcripts from urediniospores and germinated urediniospores have been examined previously, but little is known about genes expressed during host infection. Some genes involved in virulence in other rust fungi have been found to be specifically expressed in haustoria. Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.

Results: A total of 5,126 EST sequences of high quality were generated from haustoria of Pst, from which 287 contigs and 847 singletons were derived. Approximately 10% and 26% of the 1,134 unique sequences were homologous to proteins with known functions and hypothetical proteins, respectively. The remaining 64% of the unique sequences had no significant similarities in GenBank. Fifteen genes were predicted to be proteins secreted from Pst haustoria. Analysis of ten genes, including six secreted protein genes, using quantitative RT-PCR revealed changes in transcript levels in different developmental and infection stages of the pathogen.

Conclusions: The haustorial cDNA library was useful in identifying genes of the stripe rust fungus expressed during the infection process. From the library, we identified 15 genes encoding putative secreted proteins and six genes induced during the infection process. These genes are candidates for further studies to determine their functions in wheat-Pst interactions.

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Figures

Figure 1
Figure 1
Frequency distribution of sequences from the haustorial cDNA library of Puccinia striiformis f. sp. tritici belonging to the same contig or unisequence. A total of 1,134 unisequences were used in this analysis
Figure 2
Figure 2
Functional classification of the Puccinia striiformis f. sp. tritici unisequence from the haustorial cDNA library showing significant similarities to proteins in public databases
See this image and copyright information in PMC

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