[Optimization of culture conditions for crown gall of Panax quinquefolium and the content determination of their polysaccharides]

Abstract

Objective: To optimize the culture conditions and determine the content of polysaccharides in crown gall of Panax quinquefolium.

Methods: The orthogonal design was used for the optimization of culture condition (such as media, inoculum amount, pH and the content of inorganic elements in media) and their effects on the polysaccharides content.

Results: (1) The crown gall tissue could grow and produce polysaccharides in MS (Murashige & Skoog) solid medium without any hormones; (2) The time-dependent regularity of the growth amount and polysaccharides content of the crown gall tissue in MS solid medium was approached: polysaccharides content was the highest in 21 d and biomass achieved to the greatest in 24 d; (3) The polysaccharides content and growth amount were the highest when pH 5.6 of media; (4) The inoculation of 4 - 7 g (FW/flask) was comparatively advantage to the growth of the crown gall, but no effect on polysaccharides content.

Conclusion: This method can be used to accumulate the high contents of polysaccharides in crown gall cultures of P. quinquefolium.