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. 2010 Mar;43(4-5):467-72.
doi: 10.1016/j.clinbiochem.2009.12.003. Epub 2009 Dec 24.

Construction of an amperometric triglyceride biosensor using PVA membrane bound enzymes

C S Pundir  1 , Bharvi Sandeep Singh, Jagriti Narang
Affiliations

Construction of an amperometric triglyceride biosensor using PVA membrane bound enzymes

C S Pundir et al. Clin Biochem. 2010 Mar.

Abstract

Objectives: A method is described for construction of an amperometric triglyceride (TG) biosensor using PVA membrane bound enzymes.

Design and methods: A mixture of commercial lipase, glycerol kinase, glycerol-3-phosphate oxidase and horseradish peroxidase was co-immobilized onto polyvinyl alcohol (PVA) membrane through glutaraldehyde coupling. The biosensor measures current when polarized at 0.4 V.

Results: The sensor showed optimum response within 2 s at pH 7.0 and 25 degrees C. The current (mA) was in proportion to concentration of TG in the range 0.56-2.25 mM. The minimum detection limit of the method was 0.21 mM. The analytic recovery of added TG was 94.3%. Within batch and between batch coefficients of variations (CV) were <5.85% and <4.13%, respectively. A good correlation (r=0.99) was found. Among various serum substances tested, only cholesterol caused slight stimulation (20%).

Conclusions: An amperometric method was developed for determination of TG employing this enzyme electrode.

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