Association of interleukin-8 with cachexia from patients with low-third gastric cancer

Abstract

Background. Interleukin (IL)-8 has been implicated in the development of cancer cachexia. The polymorphism of IL-8 gene, which may affect the production level of IL-8, may be associated with cancer cachexia. Methods. The serum IL-8 level in our study was examined by radioimmunoassay. We also analyzed single nucleotide polymorphisms (SNPs) -251 A/T and +781 C/T of IL-8 gene, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. The serum levels of IL-8 were significantly elevated in patients with low-third gastric cancer compared with controls, and were further up-regulated in patients with cachexia than those without (Z = -3.134, P = .002). A significantly increased frequency of +781 T allele was noted in patients with cachexia (OR = 2.247, 95% CI: 1.351-3.737, P = .002). The +781 TT genotype was observed to be associated with a significantly increased risk of cachexia (OR = 3.167, 95% CI: 1.265-7.929, P = .011), and with odds ratio of 3.033 (95% CI: 1.065-8.639, P = .038) for cachexia after adjusting for potential confounding factors. Meanwhile, haplotype analysis indicated a borderline positive association between T(251)T(781) haplotype and cachexia as compared with the T(251)C(781) haplotype (OR = 4.92, 95% CI: 1.00-24.28; ,P = .053). Conclusions. IL-8 appears to be associated with cachexia from patients with low-third gastric cancer.

Figure 1

PCR-RFLP assay for analyzing the −251 A/T polymorphism of IL-8. Lanes 1 and 6 of heterozygous AT genotype showed three fragments of 173, 152, and 21 (not shown) bp; lane 4 and 7 homozygous of TT genotype showed only one fragment of 173 bp; lanes 2, 3 and 5 of homozygous AA genotype showed two fragments of 152 and 21 (not shown) bp. Lane M was loaded with 100–600 bp DNA markers.

Figure 2

PCR-RFLP assay for analyzing the +781 C/T polymorphism of IL-8. Lanes 4 and 5 of heterozygous CT genotype showed three fragments of 203, 184, and 19 (not shown) bp; lane 1 and 3 of homozygous TT genotype showed only one fragment of 203 bp; lanes 2 and 6 of homozygous CC genotype showed two fragments of 184 and 19 (not shown) bp. Lane M was loaded with 100–600 bp DNA markers.

Figure 3

Serum levels of cytokines in gastric cancer patients and healthy subjects.