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. 1991 Jan;10(1):24-6.

The production of single-stranded DNA suitable for sequencing using the polymerase chain reaction

M W Allard  1 , D L Ellsworth, R L Honeycutt
Affiliations
  • PMID: 2003916

The production of single-stranded DNA suitable for sequencing using the polymerase chain reaction

M W Allard et al. Biotechniques. 1991 Jan.

Abstract

A simple and reliable procedure for the amplification of single-stranded DNA suitable for sequencing is described. This procedure employs the polymerase chain reaction and implements modifications pertaining to the purification of the double-stranded DNA product prior to single-stranded DNA amplification. The most consistent sequencing reactions are obtained when the double-stranded DNA product is purified by centrifugation with a microconcentrator prior to single-stranded DNA amplification and the overall amount of specific primers and number of cycles used, in both single-stranded and double-stranded DNA polymerase chain reactions, are reduced.

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