Rapid polymerase chain reaction amplification using intact bacterial cells
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Rapid polymerase chain reaction amplification using intact bacterial cells
Biotechniques.
1991 Jan.
Abstract
We have demonstrated that efficient polymerase chain reaction amplifications from chromosomal DNA can be carried out using whole bacterial cells as the starting material. Cells from the liquid or solid cultures can be used directly, without any pre-treatment, thus eliminating the need for DNA isolation.
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