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. 2010 Jan 27;132(3):1018-22.
doi: 10.1021/ja9061272.

Array-based sensing of normal, cancerous, and metastatic cells using conjugated fluorescent polymers

Avinash Bajaj  1 , Oscar R Miranda, Ronnie Phillips, Ik-Bum Kim, D Joseph Jerry, Uwe H F Bunz, Vincent M Rotello
Affiliations

Array-based sensing of normal, cancerous, and metastatic cells using conjugated fluorescent polymers

Avinash Bajaj et al. J Am Chem Soc. .

Abstract

A family of conjugated fluorescent polymers was used to create an array for cell sensing. Fluorescent conjugated polymers with pendant charged residues provided multivalent interactions with cell membranes, allowing the detection of subtle differences between different cell types on the basis of cell surface features. Highly reproducible characteristic patterns were obtained from different cell types as well as from isogenic cell lines, enabling the identification of the cell type as well differentiating between normal, cancerous, and metastatic isogenic cell types with high accuracy.

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Figures

Figure 1
Figure 1
Schematic presentation of cell detection assay and interaction between polymers and cell types.
Figure 2
Figure 2
Molecular structures of the fluorescent polymers used in this study.
Figure 3
Figure 3
Jackknifed classification matrix obtained through LDA analysis for eight polymers for (a) four cell lines HeLa, MCF-7, NT2 and HepG2; (b) for three cell lines CDBgeo, TD and V14.
Figure 4
Figure 4
(a) Fluorescence responses for four different cancer cell lines HeLa (cervical), MCF7 (breast), HepG2 (liver) and NT2 (testes) using fluorescent polymers. Each value is average of six parallel measurements. (b) Canonical score plot for two factors of simplified fluorescence response patterns obtained with the fluorescent polymer arrays
Figure 5
Figure 5
(a) Fluorescence responses for three isogenic cell lines CDBgeo, TD cell and V14 using polymer arrays. Each value is average of six parallel measurements. (b) Canonical score plot for first two factors of simplified fluorescence response patterns obtained with polymer arrays against different mammalian cell types. The canonical scores were calculated by LDA for identification of three cell lines.
See this image and copyright information in PMC

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