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. 2008 Oct;40(5):230-4.
doi: 10.4103/0253-7613.44156.

Antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India

Viren A Javiya  1 , Somsuvra B Ghatak, Kamlesh R Patel, Jagruti A Patel
Affiliations

Antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India

Viren A Javiya et al. Indian J Pharmacol. 2008 Oct.

Abstract

Objectives: The present study was undertaken to assess the antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India. Due to significant changes in microbial genetic ecology, as a result of indiscriminate use of anti-microbials, the spread of anti-microbial resistance is now a global problem.

Materials and methods: Out of 276 culture positive samples, 56 samples of Pseudomonas aeruginosa were examined and 10 different types of specimen were collected. Microbial sensitivity testing was done using disk diffusion test with Pseudomonas species NCTC 10662, as per CLSI guidelines.

Results: The highest number of Pseudomonas infections was found in urine, followed by pus and sputum. Pseudomonas species demonstrated marked resistance against monotherapy of penicillins, cephalosporins, fluoroquinolones, tetracyclines and macrolides. Only combination drugs like Ticarcillin + Clavulanic acid, Piperacillin + Tazobactum, Cefoperazone + Sulbactum, Cefotaxime + Sulbactum, Ceftriaxome + Sulbactum and monotherapy of amikacin showed higher sensitivity to Pseudomonas infections; however, the maximum sensitivity was shown by the Carbapenems.

Conclusion: From the present study, we conclude that urinary tract infection was the most common hospital acquired infection. Also, co-administration of beta -lactamase inhibitors markedly expanded the anti-microbial sensitivity of semi-synthetic penicillins and cephalosporins. The aminoglycoside group of antibiotics - amikacin - demonstrated maximum sensitivity against pseudomonas species. Therefore, use of amikacin should be restricted to severe nosocomial infections, in order to avoid rapid emergence of resistant strains. Periodic susceptibility testing should be carried out over a period of two to three years, to detect the resistance trends. Also, a rational strategy on the limited and prudent use of anti-Pseudomonal agents is urgently required.

Keywords: Antimicrobial susceptibility; Pseudomonas aeruginosa; carbapenem sensitivity; combination antibiotics; disk diffusion technique.

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Figures

Figure 1a
Figure 1a
Antibiotic resistance patterns of P. aeruginosa against penicillin group of antibiotics. Ampicillin, amoxicillin, ticarcillin and piperacillin in combination with sulbactum, clavulanic acid and tazobactum demonstrated significantly higher antibacterial activity (P≤0.001, Chi-square test) against P. aeruginosa when compared to the monotherapy of respective antibiotics
Figure 1b
Figure 1b
Antibiotic resistance patterns of P. aeruginosa against cephalosporin group of antibiotics. Cefoperazone, cefotaxime and ceftriaxone in combination with sulbactum demonstrated significantly higher antibacterial activity (P≤0.001, Chi-square test) when compared to monotherapy of respective antibiotics
Figure 1c
Figure 1c
Antibiotic resistance patterns of P. aeruginosa against carbapenem group of antibiotics. Imipenem and meropenem demonstrated significantly higher antibacterial activity (P≤0.001, Chi-square test) when compared to aztreonem
See this image and copyright information in PMC

References

    1. Chastre J, Trouillet JL. Problem pathogens (Pseudomonas aeruginosa and Acinetobacter) Semin Respir Infect. 2000;15:287–98. - PubMed
    1. Obritsch MD, Fish DN, McLaren R, Jung R. National Surveillance of Antimicrobial Resistance in Pseudomonas aeruginosa isolates obtained from Intensive Care Unit Patients from 1993 to 2002. Antimicrob Agents Chem. 2004;48:4606–10. - PMC - PubMed
    1. Gençer S, Ak O, Benzonana N, Batirel A, Ozer S. Susceptibility pattern and cross resistances of antibiotics against Pseudomonas aeruginosa in a teaching hospital of Turkey. Ann Clin Microbiol Antimicrob. 2002;1:2. - PMC - PubMed
    1. National Committee for Clinical Laboratory Standards. Approved standard M2 A7 NCCLS. Villanova, PA: 1995. Performance standards for antimicrobial disk susceptibility tests; p. 15.
    1. Das RN, Chandrasekhar TS, Joshi HS, Gurung M, Shrestha N, Shivananda PG. Frequency and susceptibility profile of pathogens causing urinary tract infections at a tertiary care hospital in western Nepal. Singapore Med J. 2006;47:281–5. - PubMed

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