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. 2010 Sep 1;127(6):1493-6.
doi: 10.1002/ijc.25136.

Immunological detection of viral large T antigen identifies a subset of Merkel cell carcinoma tumors with higher viral abundance and better clinical outcome

Kishor Bhatia, James J Goedert, Rama Modali, Liliana Preiss, Leona W Ayers

Immunological detection of viral large T antigen identifies a subset of Merkel cell carcinoma tumors with higher viral abundance and better clinical outcome

Kishor Bhatia et al. Int J Cancer. .
No abstract available

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Figures

Figure 1
Figure 1
Figure 1A: Immunohistochemical staining of MCC was performed for the detection of expression of retinoblastoma protein (pRb) using the commercially available antibody from Novocastra (clone 13A10) and for the detection of the LTA encoded by MCPyV using the CM2B4 mAb obtained as a gift from Dr. Patrick Moore and Yuan Chang (University of Pittsburgh). These antibodies were applied to formalin-fixed paraffin-embedded Merkel cell carcinoma tumor core samples in an MCC tissue microarray. MCPyV copies per cell (CPC) were determined by real time PCR using Taqman technology. Details of the PCR assay have been described previously. MCPyV CPC associates with (row A) expression of MCPyV LTA using CM2B4 mAb and (row B) expression of pRb. Figure 1B: Correlation between overall survival (Kaplan Meier) and the presence of MCPyV LTA by CM2B4 mAb staining demonstrates that patients with LTA positive tumors survived longer following a diagnosis of MCC than patients with LTA negative tumors. By Cox relative hazard (RH) analysis, likelihood of survival was lower per level (HR=0.35, CI=0.16–0.77, P=0.009). The number of cases in each category is: LTA positive, viral positive, 9; LTA negative, viral positive, 8; and LTA negative, viral negative, 6. Patients with viral positive tumors tended to be younger (P=0.07) but did not differ by sex. Additional characteristics of these patients have been described previously.
Figure 1
Figure 1
Figure 1A: Immunohistochemical staining of MCC was performed for the detection of expression of retinoblastoma protein (pRb) using the commercially available antibody from Novocastra (clone 13A10) and for the detection of the LTA encoded by MCPyV using the CM2B4 mAb obtained as a gift from Dr. Patrick Moore and Yuan Chang (University of Pittsburgh). These antibodies were applied to formalin-fixed paraffin-embedded Merkel cell carcinoma tumor core samples in an MCC tissue microarray. MCPyV copies per cell (CPC) were determined by real time PCR using Taqman technology. Details of the PCR assay have been described previously. MCPyV CPC associates with (row A) expression of MCPyV LTA using CM2B4 mAb and (row B) expression of pRb. Figure 1B: Correlation between overall survival (Kaplan Meier) and the presence of MCPyV LTA by CM2B4 mAb staining demonstrates that patients with LTA positive tumors survived longer following a diagnosis of MCC than patients with LTA negative tumors. By Cox relative hazard (RH) analysis, likelihood of survival was lower per level (HR=0.35, CI=0.16–0.77, P=0.009). The number of cases in each category is: LTA positive, viral positive, 9; LTA negative, viral positive, 8; and LTA negative, viral negative, 6. Patients with viral positive tumors tended to be younger (P=0.07) but did not differ by sex. Additional characteristics of these patients have been described previously.
See this image and copyright information in PMC

References

    1. Feng H, Shuda M, Chang Y, Moore PS. Clonal Integration of a polyomavirus in human Merkel cell carcinoma. Science. 2008;319:1096–1100. - PMC - PubMed
    1. Shuda M, Arora R, Kwun HJ, Feng H, Sarid R, Fernández-Figueras MT, Tolstov Y, Gjoerup O, Mansukhani MM, Swerdlow SH, Chaudhary PM, Kirkwood JM, et al. Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors. Int J Cancer. 2009;125:1243–1249. - PMC - PubMed
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