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. 2010 Feb;22(2):125-32.
doi: 10.3109/08958370902953910.

Whole-body inhalation exposure to 1-bromopropane suppresses the IgM response to sheep red blood cells in female B6C3F1 mice and Fisher 344/N rats

Stacey E Anderson  1 , Albert E Munson, Leon F Butterworth, Dori Germolec, Daniel L Morgan, Joseph A Roycroft, Jeffrey Dill, B J Meade
Affiliations

Whole-body inhalation exposure to 1-bromopropane suppresses the IgM response to sheep red blood cells in female B6C3F1 mice and Fisher 344/N rats

Stacey E Anderson et al. Inhal Toxicol. 2010 Feb.

Abstract

1-Bromopropane (1-BP) is categorized as a high-production-volume chemical and is currently used in the manufacture of pharmaceuticals, pesticides, and other chemicals. Its usage is estimated to be around 5 million pounds per year, resulting in the potential for widespread exposure in the workplace. Case reports and animal studies have suggested exposure to this compound may cause adverse reproductive and neurological effects. Using a battery of immunological assays, the immunotoxicity of 1-BP after whole body inhalation exposure in both mice and rats was evaluated. Significant decreases in the spleen immunoglobulin (Ig) M response to sheep red blood cells (SRBC) were observed in both mice (125-500 ppm) and rats (1000 ppm) after exposure to 1-BP for 10 wk. In addition, total spleen cells and T cells were significantly decreased after approximately 4 wk of 1-BP exposure in both mice (125-500 ppm) and rats (1000 ppm). No change in natural killer (NK) cell activity was observed. The observed alterations in spleen cellularity, phenotypic subsets, and impairment of humoral immune function across species raise further concern about human exposure to 1-BP and demonstrate the need for additional investigations into potential adverse health effects.

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Figures

Figure 1
Figure 1. Inhalation Exposure to 1-BP Suppresses the Spleen IgM Response to SRBC in B6C3F1 Mice
The IgM response to SRBC/106 splenocytes (A, C) and IgM response to SRBC/spleen (B, D) is illustrated after expose to 1-bromopropane for 4 and 10 weeks. Values represent the mean ± SE derived from eight animals (5 animals for 500 ppm dose) in each group. *p < 0.05 vs. air control.
Figure 2
Figure 2. Inhalation Exposure to 1-BP Decreases Spleen Cellularity in B6C3F1 Mice
Spleen cellularity is illustrated after exposure of female B6C3F1 to 1-bromopropane for 4 (A) and 10 weeks (B). Values represent the mean ± SE derived from eight animals (5 animals for 500 ppm dose) in each group. *p < 0.05 vs. air control
Figure 3
Figure 3. Inhalation Exposure to 1-BP Suppresses the IgM Response to SRBC in Fisher 344 Rats
The IgM response to SRBC/106 splenocytes (A) and IgM response to SRBC/spleen (B) is illustrated after exposure to 1-bromopropane for 10 weeks. Values represent the mean ± SE derived from eight animals in each group. *p < 0.05 vs. air control.
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References

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