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. 2010 Mar;48(3):1012-3.
doi: 10.1128/JCM.01774-09. Epub 2009 Dec 30.

Further characterization of proteolytic Clostridium botulinum type A5 reveals that neurotoxin formation is unaffected by loss of the cntR (botR) promoter sigma factor binding site

Andrew T Carter, David R Mason, Kathie A Grant, Giovanna Franciosa, Paulo Aureli, Michael W Peck

Further characterization of proteolytic Clostridium botulinum type A5 reveals that neurotoxin formation is unaffected by loss of the cntR (botR) promoter sigma factor binding site

Andrew T Carter et al. J Clin Microbiol. 2010 Mar.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Position of a 76-bp deletion identified in the neurotoxin gene cluster of proteolytic C. botulinum type A5 strain H04402 065. Neurotoxins and associated genes are shaded. Prime symbols denote a partial gene. Upper part of figure represents coding regions spanning 18.2 kb of a chromosomal DNA sequence (GenBank accession number GQ477161). The −35 and −10 binding sites for the CntR/RNA polymerase complex are indicated for each gene that is positively regulated by CntR. Alternative nomenclature for neurotoxin cluster genes is as follows: cntC (ha34), cntD (ha17), cntE (ha70), cntR (botR), cntB (ntnh), cntA/A5 (bont/A5), cntA/B3′ (bont/B3′).
FIG. 2.
FIG. 2.
Growth and neurotoxin formation by proteolytic C. botulinum type A5 strain H04402 065 (squares) and type A1 strain ATCC 3502 (triangles). Closed symbols represent the viable count (left y axis); open symbols represent the neurotoxin concentration in culture supernatant (right y axis).
See this image and copyright information in PMC

Comment in

References

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