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. 2010 Jan;11(1):10-6.
doi: 10.1631/jzus.B0900163.

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Tie-lian Liu  1 , Jing-cheng Miao, Wei-hua Sheng, Yu-feng Xie, Quan Huang, Yun-bo Shan, Ji-cheng Yang
Affiliations

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Tie-lian Liu et al. J Zhejiang Univ Sci B. 2010 Jan.

Abstract

Objective: To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility.

Methods: The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor (VEGF) expression of ECV304 cells, and VEGF, angiopoietin-1 (Ang-1), platelet-derived growth factor (PDGF) and fibroblast growth factor 2 (FGF2) expression of WI-38 cells were assessed by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, viable cell counting, flow cytometry (FCM), and enzyme-linked immunosorbant assay (ELISA).

Results: We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells.

Conclusion: The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.

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Figures

Fig. 1
Fig. 1
The results of flow cytometric analysis (a) SF; (b) PVC; (c) PE; (d) Control
Fig. 1
Fig. 1
The results of flow cytometric analysis (a) SF; (b) PVC; (c) PE; (d) Control
Fig. 1
Fig. 1
The results of flow cytometric analysis (a) SF; (b) PVC; (c) PE; (d) Control
Fig. 1
Fig. 1
The results of flow cytometric analysis (a) SF; (b) PVC; (c) PE; (d) Control
Fig. 2
Fig. 2
The morphologies (×200) (a) and the growth curves (b) of ECV304 cells on different materials
Fig. 2
Fig. 2
The morphologies (×200) (a) and the growth curves (b) of ECV304 cells on different materials
Fig. 3
Fig. 3
The levels of VEGF secreted by ECV304 cells on different materials
Fig. 4
Fig. 4
The secretion levels of VEGF, Ang-1, FGF2 and PDGF by WI-38 cells on SF
See this image and copyright information in PMC

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