doi: 10.1016/j.ab.2009.12.036.
Epub 2010 Jan 4.
A high recovery microsampling device based on a microdialysis probe for peptide sampling
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- PMID: 20045671
- DOI: 10.1016/j.ab.2009.12.036
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A high recovery microsampling device based on a microdialysis probe for peptide sampling
Anal Biochem.
.
Abstract
A high recovery microsampling probe based on microdialysis was devised. The new probe showed a high recovery (100%) of peptides in vitro at different perfusion flow rates (0.1-1.0 microl/min). At a high flow rate, 1.0 microl/min, a 10-fold increased in recovery of peptides compared to the conventional microdialysis probe was achieved. A probe made of a low molecular weight cutoff membrane is suitable for filtering off proteins. The new probe can be a useful tool for high recovery of peptides from living tissues.
Copyright 2010 Elsevier Inc. All rights reserved.
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