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. 2009 Oct;73(4):283-91.

Prevalence of bovine viral diarrhea virus (BVDV) in persistently infected cattle and BVDV subtypes in affected cattle in beef herds in south central United States

Robert W Fulton  1 , Evan M Whitley, Bill J Johnson, Julia F Ridpath, Sanjay Kapil, Lurinda J Burge, Billy J Cook, Anthony W Confer
Affiliations

Prevalence of bovine viral diarrhea virus (BVDV) in persistently infected cattle and BVDV subtypes in affected cattle in beef herds in south central United States

Robert W Fulton et al. Can J Vet Res. 2009 Oct.

Abstract
in English, French

The prevalence of bovine viral diarrhea virus (BVDV) in persistently infected (PI) cattle in beef breeding herds was determined using 30 herds with 4530 calves. The samples were collected by ear notches and tested for BVDV antigens using immunohistochemistry (IHC) and antigen capture enzyme-linked immunosorbent assay (ACE). Animals with initial positives on both IHC and ACE were sampled again using both tests and serums were collected for viral propagation and sequencing of a viral genomic region, 5'-untranslated region (5'-UTR) for viral subtyping. Samples were also collected from the dams of PI calves. There were 25 PI calves from 4530 samples (0.55%) and these PI calves were from 5 of the 30 herds (16.7%). Two herds had multiple PI calves and 3 herds had only 1 PI calf. Only 1 of the 25 dams with a PI calf was also PI (4.0%). The subtype of all the PI isolates was BVDV1b. Histories of the ranches indicated 23 out of 30 had herd additions of untested breeding females. Twenty-four of the 30 herds had adult cowherd vaccinations against BVDV, primarily using killed BVDV vaccines at pregnancy examination.

La prévalence du virus de la diarrhée virale bovine (BVDV) chez des bovins infectés de manière persistante (PI) dans des troupeaux reproducteurs de bovins de boucherie a été déterminée chez 4530 veaux dans 30 troupeaux. Les échantillons ont été prélevés par encoches d’oreille et testés pour les antigènes du BVDV par immunohistochimie (IHC) et par test de capture antigénique immuno-enzymatique (ACE). Les animaux trouvés positifs par IHC et ACE lors du premier échantillonnage ont été éprouvés de nouveau à l’aide des deux tests, et des sérums ont été prélevés pour culture et séquençage d’une région génomique virale, la région 5′-non-traduite (5′-UTR), pour sous-typage viral. Des échantillons ont également été prélevés sur les mères des veaux PI. Il y avait 25 veaux PI parmi les 4530 échantillons (0,55 %) et ces veaux PI provenaient de 5 des 30 troupeaux (16,7 %). Deux troupeaux avaient plus de 1 veaux PI et 3 troupeaux avaient seulement 1 veau PI. Seulement 1 des 25 mères avec un veau PI était également PI (4,0 %). Le sous-type de tous les isolats était du BVDV1b. Le signalement des différentes fermes indiquait que 23 des 30 avaient fait des ajouts de femelles reproductrices non-éprouvées. Vingt-quatre des 30 fermes avaient un programme de vaccination des vaches adultes contre le BVDV, utilisant principalement un vaccin BVDV tué au moment des examens de gestation.

(Traduit par Docteur Serge Messier)

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Figures

Figure 1
Figure 1
Dendogram showing subtyping of bovine viral diarrhea virus (BVDV) strains from persistently infected (PI) cattle.
See this image and copyright information in PMC

References

    1. Baker JC. The clinical manifestations of bovine viral diarrhea infection. Vet Clin North Am Food Anim Pract. 1995;11:425–445. - PubMed
    1. Fulton RW, Ridpath JF, Ore S, et al. Bovine viral diarrhea virus (BVDV) subgenotypes in diagnostic laboratory accessions: Distribution of BVDV1a, 1b, and 2a subgenotypes. Vet Microbiol. 2005;111:35–40. - PubMed
    1. Flores EF, Ridpath JF, Weiblen R, et al. Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: Evidence for a subgenotype within BVDV-2. Virus Res. 2002;87:51–60. - PubMed
    1. Fulton RW, Saliki JT, Burge LJ, et al. Neutralizing antibodies to type 1 and 2 bovine viral diarrhea viruses: Detection by inhibition of viral cytopathology and infectivity by immunoperoxidase assay. Clin Diagn Lab Immunol. 1997;4:380–383. - PMC - PubMed
    1. Fulton RW, Ridpath JF, Confer AW, et al. Bovine viral diarrhea antigenic diversity: Impact on disease and vaccination programmes. Biologicals. 2003;31:89–95. - PubMed

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