Little vector, big gene transduction: fragmented genome reassembly of adeno-associated virus

Affiliations

Comment

Matthew L Hirsch et al. Mol Ther. 2010 Jan.

. 2010 Jan;18(1):6-8.

doi: 10.1038/mt.2009.280.

¹ UNC Gene Therapy Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

No abstract available

Figures

Effect of genome size on AAV vector packaging.
Wu Z, Yang H, Colosi P. Wu Z, et al. Mol Ther. 2010 Jan;18(1):80-6. doi: 10.1038/mt.2009.255. Epub 2009 Nov 10. Mol Ther. 2010. PMID: 19904234 Free PMC article.
Characterization of genome integrity for oversized recombinant AAV vector.
Dong B, Nakai H, Xiao W. Dong B, et al. Mol Ther. 2010 Jan;18(1):87-92. doi: 10.1038/mt.2009.258. Epub 2009 Nov 10. Mol Ther. 2010. PMID: 19904236 Free PMC article.
Evidence for the failure of adeno-associated virus serotype 5 to package a viral genome > or = 8.2 kb.
Lai Y, Yue Y, Duan D. Lai Y, et al. Mol Ther. 2010 Jan;18(1):75-9. doi: 10.1038/mt.2009.256. Epub 2009 Nov 10. Mol Ther. 2010. PMID: 19904238 Free PMC article.

1. McCarty DM, Young SM., Jr, and , Samulski RJ. Integration of adeno-associated virus (AAV) and recombinant AAV vectors. Annu Rev Genet. 2004;38:819–845. - PubMed
1. Li W, Asokan A, Wu Z, Van Dyke T, DiPrimio N, Johnson JS.et al. (2008Engineering and selection of shuffled AAV genomes: a new strategy for producing targeted biological nanoparticles Mol Ther 161252–1260. - PubMed
1. Maheshri N, Koerber JT, Kaspar BK., and , Schaffer DV. Directed evolution of adeno-associated virus yields enhanced gene delivery vectors. Nat Biotechnol. 2006;24:198–204. - PubMed
1. Wu Z, Asokan A, Samulski RJ. Adeno-associated virus serotypes: vector toolkit for human gene therapy. Mol Ther. 2006;14:316–327. - PubMed
1. Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D.et al. (2008Serotype-dependent packaging of large genes in adeno-associated viral vectors results in effective gene delivery in mice J Clin Invest 1181955–1964. - PMC - PubMed