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. 1991 Jan;22(1):19-22.
doi: 10.1016/0165-022x(91)90077-a.

Microassay for DNA methyltransferase

R L Adams  1 , A Rinaldi, C Seivwright
Affiliations

Microassay for DNA methyltransferase

R L Adams et al. J Biochem Biophys Methods. 1991 Jan.

Abstract

A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.

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