Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Dec 1;65(Pt 12):1261-3.
doi: 10.1107/S1744309109038007. Epub 2009 Nov 27.

Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA

Vinod B Agarkar  1 , Nigar D Babayeva, Angie Rizzino, Tahir H Tahirov
Affiliations

Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA

Vinod B Agarkar et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Ets proteins are transcription factors that activate or repress the expression of genes that are involved in various biological processes, including cellular proliferation, differentiation, development, transformation and apoptosis. Like other Ets-family members, Elf3 functions as a sequence-specific DNA-binding transcriptional factor. A mouse Elf3 C-terminal fragment (amino-acid residues 269-371) containing the DNA-binding domain has been crystallized in complex with mouse type II TGF-beta receptor promoter (TbetaR-II) DNA. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 42.66, b = 52, c = 99.78 A, and diffracted to a resolution of 2.2 A.

PubMed Disclaimer

Figures

Figure 1
Figure 1
(a) A photomicrograph of the crystals of mElf3269–371–mTβRII dsDNA. (b) EMSA. Lane 1, DNA. Lane 2, initial sample. Lane 3, washed and dissolved crystals.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Chang, C. H., Scott, G. K., Kuo, W. L., Xiong, X., Suzdaltseva, Y., Park, J. W., Sayre, P., Erny, K., Collins, C., Gray, J. W. & Benz, C. C. (1997). Oncogene, 14, 1617–1622. - PubMed
    1. Chang, J., Lee, C., Hahm, K. B., Yi, Y., Choi, S. G. & Kim, S. J. (2000). Oncogene, 19, 151–154. - PubMed
    1. Choi, S. G., Yi, Y., Kim, Y. S., Kato, M., Chang, J., Chung, H. W., Hahm, K. B., Yang, H. K., Rhee, H. H., Bang, Y. J. & Kim, S. J. (1998). J. Biol. Chem.273, 110–117. - PubMed
    1. Dittmer, J. & Nordheim, A. (1998). Biochim. Biophys. Acta, 1377, F1–F11. - PubMed
    1. Donaldson, L. W., Petersen, J. M., Graves, B. J. & McIntosh, L. P. (1996). EMBO J.15, 125–134. - PMC - PubMed

Publication types

MeSH terms