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. 2009 Dec 23;10(12):5513-5527.
doi: 10.3390/ijms10125513.

Antioxidant activity of a red lentil extract and its fractions

Ryszard Amarowicz  1 , Isabell Estrella  2 , Teresa Hernández  2 , Montserrat Dueñas  3 , Agnieszka Troszyńska  1 , Agnieszka Kosińska  1 , Ronald B Pegg  4
Affiliations

Antioxidant activity of a red lentil extract and its fractions

Ryszard Amarowicz et al. Int J Mol Sci. .

Abstract

Phenolic compounds were extracted from red lentil seeds using 80% (v/v) aqueous acetone. The crude extract was applied to a Sephadex LH-20 column. Fraction 1, consisting of sugars and low-molecular-weight phenolics, was eluted from the column by ethanol. Fraction 2, consisting of tannins, was obtained using acetone-water (1:1; v/v) as the mobile phase. Phenolic compounds present in the crude extract and its fractions demonstrated antioxidant and antiradical activities as revealed from studies using a beta-carotene-linoleate model system, the total antioxidant activity (TAA) method, the DPPH radical-scavenging activity assay, and a reducing power evaluation. Results of these assays showed the highest values when tannins (fraction 2) were tested. For instance, the TAA of the tannin fraction was 5.85 micromol Trolox eq./mg, whereas the crude extract and fraction 1 showed 0.68 and 0.33 micromol Trolox eq./mg, respectively. The content of total phenolics in fraction 2 was the highest (290 mg/g); the tannin content, determined using the vanillin method and expressed as absorbance units at 500 nm per 1 g, was 129. There were 24 compounds identified in the crude extract using an HPLC-ESI-MS method: quercetin diglycoside, catechin, digallate procyanidin, and p-hydroxybenzoic were the dominant phenolics in the extract.

Keywords: HPLC-ESI-MS; free radicals; phenolic compounds; red lentils; tannins.

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Figures

Figure 1.
Figure 1.
UV spectra of a red lentil acetonic extract and its fractions (1–extract; 2–fraction 1; and 3–fraction 2).
Figure 2.
Figure 2.
Antioxidant activity of a red lentil acetonic extract and its fractions in a β-carotene-linoleate model system, as measured by changes in absorbance at 470 nm.
Figure 3.
Figure 3.
Reducing power of a red lentil acetonic extract end its fractions, as measured by changes in optical density at 700 nm.
Figure 4.
Figure 4.
Scavenging effect of a red lentil acetonic extract and its fractions on the DPPH radical, as measured by changes in absorbance at 517 nm.
See this image and copyright information in PMC

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