Effect of the extract made from Cochinchina momordica seeds on the humoral immune responses of mice to a commercial foot-and-mouth disease vaccine (serotypes O and Asia 1)

Abstract

The extract from ECMS was investigated for its effect on the humoral immune responses to foot-and-mouth disease vaccination. Fifty-six mice were randomly divided into seven groups with eight animals in each. Mice in groups 5 to 7 were subcutaneously (s.c.) injected with 0.5 mg DEX daily for 4 days to induce immunosuppression. The animals were then orally given ECMS (200 microg in 250 microl saline) in groups 3 and 6 or 250 microl saline in group 2, or s.c. injected with ECMS (50 microg in 100 microl saline) in groups 4 and 7 or 100 microl saline in group 5. After that, the animals in groups 2 to 7 were s.c. immunized twice with 100 microl of commercial oil-adjuvanted bivalent FMDV vaccine (serotypes O and Asia 1) at intervals of 21 days. Mice in group 1 received injection of 100 microl saline only. After 2 weeks, blood was sampled to determine FMDV-specific IgG and isotype IgG1, IgG2a, IgG2b and IgG3. Results indicated that oral administration or s.c. injection of ECMS augmented responses of specific IgG and most IgG isotypes. Giving ECMS tended to enhance serum-specific IgG and IgG isotype responses of mice immunosuppressed by s.c. injection of DEX. Considering the safety and immunomodulatory effect of ECMS in both normal and immunosuppressed mice demonstrated in the present study, this extract deserves further investigation to evaluate its potential in improving FMD vaccination in farm animals such as pigs, sheep and cattle.