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Review
. 2010 Mar;10(6):1284-96.
doi: 10.1002/pmic.200900418.

MS-driven protease substrate degradomics

Francis Impens  1 , Niklaas Colaert, Kenny Helsens, Kim Plasman, Petra Van Damme, Joël Vandekerckhove, Kris Gevaert
Affiliations
Review

MS-driven protease substrate degradomics

Francis Impens et al. Proteomics. 2010 Mar.

Erratum in

  • Proteomics. 2010 Jul;10(13):2560

Abstract

Proteolytic processing has recently received increased attention in the field of signal propagation and cellular differentiation. Because of its irreversible nature, protein cleavage has been associated with committed steps in cell function. One aspect of protease biology that boomed the past few years is the detailed characterization of protease substrates by both shotgun as well as targeted MS-driven proteomics techniques. The most promising techniques are discussed in this review and we further elaborate on the bioinformatics challenges that accompany mainly qualitative, MS-driven protease substrate degradome studies.

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