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. 1991 Apr;11(4):2328-32.
doi: 10.1128/mcb.11.4.2328-2332.1991.

Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation

L E Brown  1 , S L Sprecher, L R Keller
Affiliations

Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation

L E Brown et al. Mol Cell Biol. 1991 Apr.

Abstract

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

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