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. 2009 Jan;29(1):23-7.
doi: 10.4103/0973-3930.50711.

Detection of tissue origin of a 43 kDa diabetogenic protein from alloxan-induced diabetic rats

Shivkumar D Chauhan  1 , Nirmalendu M Nath, Vinay K Tule
Affiliations

Detection of tissue origin of a 43 kDa diabetogenic protein from alloxan-induced diabetic rats

Shivkumar D Chauhan et al. Int J Diabetes Dev Ctries. 2009 Jan.

Abstract

Background: Earlier, we had found high levels of circulating immune complexes (CICs) in the serum of type 2 diabetes mellitus patients along with a novel 43 kDa protein.

Methods: Different tissues of alloxan-induced, diabetic, male albino rats (200-250 g in body weight) were collected for the present study. Tissue proteins were isolated and separated by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). A primary cell culture of polymorphonuclear neutrophils (PMNs) was used to evaluate the effects of the diabetogenic protein. Cell proliferative index, oxidant/antioxidant status, and ion-transporting ability were chosen as study parameters.

Results: SDS-PAGE of different tissues shows that the diabetic liver alone was the only tissue that contained the 43 kDa protein band compared to the normal liver. In vitro effects of the new liver protein on PMNs include significantly decreased cell proliferative activity, increased free radical levels, and decreased levels of antioxidant enzymes as well as ionic transporters. The new liver protein also exhibited protease activity when compared with standard trypsin.

Conclusions: This study concluded that a novel 43 kDa protein obtained from the livers of alloxan-induced diabetic rats shows protease activity as well as antiproliferative activity. Also, this protein may act as a diabetogenic factor as it elicited a significantly gross elevation in the oxidant status level as well as in the levels of lysosomal enzymes and a decrease in the levels of antioxidative enzymes and ionic transporters of PMNs.

Keywords: Alloxan diabetes; diabetogenic factor; ionic transporters; oxidant/antioxidant status; polymorphonuclear neutrophils.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
SDS-PAGE electrophoresis pattern of normal and alloxan-induced diabetic liver proteins
Figure 2
Figure 2
Effect of hepatic 43 kDa protein obtained from alloxan-induced diabetic rats on PMN growth rate; results expressed as mean ± SD of 7 experiments
See this image and copyright information in PMC

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