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. 2009:2009:658965.
doi: 10.4061/2009/658965. Epub 2009 Dec 22.

The Impact of Adenosine Fast Induction of Myocardial Arrest during CABG on Myocardial Expression of Apoptosis-Regulating Genes Bax and Bcl-2

Ahmed Shalaby  1 , Timo Rinne, Otso Järvinen, Juha Latva-Hirvelä, Kristiina Nuutila, Antti Saraste, Jari Laurikka, Helena Porkkala, Pekka Saukko, Matti Tarkka
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The Impact of Adenosine Fast Induction of Myocardial Arrest during CABG on Myocardial Expression of Apoptosis-Regulating Genes Bax and Bcl-2

Ahmed Shalaby et al. Cardiol Res Pract. 2009.

Abstract

Background. We studied the effect of fast induction of cardiac arrest with denosine on myocardial bax and bcl-2 expression. Methods and Results. 40 elective CABG patients were allocated into two groups. The adenosine group (n = 20) received 250 mug/kg adenosine into the aortic root followed by blood potassium cardioplegia. The control group received potassium cardioplegia in blood. Bcl-2 and bax were measured. Bax was reduced in the postoperative biopsies (1.38 versus 0.47, P = .002) in the control group. Bcl-2 showed a reducing tendency (0.14 versus 0.085, P = .07). After the adenosine treatment, the expression of both bax (0.52 versus 0.59, P = .4) and bcl-2 (0.104 versus 0.107, P = .4) remained unaltered after the operation. Conclusion. Open heart surgery is associated with rapid reduction in the expression of apoptosis regulating genes bax and bcl-2. Fast Adenosine induction abolished changes in their expression.

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Figures

Figure 1
Figure 1
Baseline versus the postoperative expressions of bax and bcl2 in adenosine treated and the control groups. Gene expression is normalized to the same endogenous gene. Before and After refer to the timing of taking the biopsies (Before; before cross clamp application and After; after cross clamp removal and just before CPB discontinuation).
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