Review
doi: 10.1007/s12026-009-8148-z.
Transcriptional target-based expression cloning of immunoregulatory molecules
Affiliations
- PMID: 20069388
- PMCID: PMC2892239
- DOI: 10.1007/s12026-009-8148-z
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Review
Transcriptional target-based expression cloning of immunoregulatory molecules
Immunol Res.
2010 Jul.
Abstract
Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.
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