Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) in diverse clinical specimens by the BD GeneOhm MRSA assay and comparison with culture
- PMID: 20071545
- PMCID: PMC2832448
- DOI: 10.1128/JCM.01990-09
Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) in diverse clinical specimens by the BD GeneOhm MRSA assay and comparison with culture
Abstract
The efficacy of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay was assessed by analyzing nasal swabs and swabs from other body sites for the presence of MRSA in a low-prevalence area. From 681 patients with a high risk for MRSA carriage, 1,601 specimens were collected and transported in Amies agar. After discordant analysis, the sensitivity, specificity, positive predictive value, and negative predictive value of the BD GeneOhm MRSA assay were 84.3%, 99.2%, 88.4%, and 98.9%, respectively, compared to culture.
References
-
- Altmann, D. G., and R. G. Newcombe. 2000. Proportions and their differences, and diagnostic tests, p. 46-48, 106-107. In D. G. Altmann, D. Machin, T. N. Bryant, and M. J. Gardner (ed.), Statistics with confidence, 2nd ed. BMJ Books, Bristol, United Kingdom.
-
- Bartels, M. D., K. Boye, S. M. Rohde, A. R. Larsen, H. Torfs, P. Bouchy, R. Skov, and H. Westh. 2009. A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay. J. Clin. Microbiol. 47:1524-1527. - PMC - PubMed
-
- Bishop, E. J., E. A. Grabsch, S. A. Ballard, B. Mayall, S. Xie, R. Martin, and M. L. Grayson. 2006. Concurrent analysis of nose and groin swab specimens by the IDI-MRSA PCR assay is comparable to analysis by individual-specimen PCR and routine culture assays for detection of colonization by methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 44:2904-2908. - PMC - PubMed
-
- Blanc, D. S., D. Pittet, C. Ruef, A. F. Widmer, K. Mühlemann, C. Petignat, S. Harbarth, R. Auckenthaler, J. Bille, R. Frei, R. Zbinden, R. Peduzzi, V. Gaia, H. Khamis, E. Bernasconi, and P. Francioli. 2002. Epidemiology of methicillin-resistant Staphylococcus aureus: results of a nation-wide survey in Switzerland. Swiss Med. Wkly. 132:223-229. - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Medical