doi: 10.1364/AO.8.001055.
The examination of multicomponent systems in biological materials by means of a rapid scanning photometer
- PMID: 20072373
- DOI: 10.1364/AO.8.001055
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The examination of multicomponent systems in biological materials by means of a rapid scanning photometer
Appl Opt.
.
Abstract
Only photometers scanning a large range of wavelengths provide the amount of information which is necessary to analyze multicomponent systems in a general form. After a description of a rapid scanning photometer and a discussion of the recording problems, the mathematical method is described which allows an optimal analysis of the concentrations of the single components of a multicomponent system. The error involved in this procedure is demonstrated by the calculation of the four nucleotides in a mixture. The long range photometry has been successfully applied to other problems such as measurements by reflected light and analyses of inhomogeneously distributed substances in living tissues.
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