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. 1991 Feb;29(2):393-4.
doi: 10.1128/jcm.29.2.393-394.1991.

Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi

A Espinel-Ingroff  1 , T M Kerkering
Affiliations

Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi

A Espinel-Ingroff et al. J Clin Microbiol. 1991 Feb.

Abstract

Homogeneous inoculum suspensions of 29 isolates of clinically important filamentous fungi were adjusted with a spectrophotometer (530 nm) to obtain standardized preparations containing 1 x 10(6) to 5 x 10(6) CFU/ml. Colony counts (CFU per milliliter) of 1 x 10(6) to 5 x 10(6) were achieved on three different days for isolates of Aspergillus spp., Pseudallescheria boydii, and Sporothrix schenckii (80% +/- 2% transmission), and colony counts of 7 x 10(5) to 2.9 x 10(6) (70% +/- 2% transmission) were achieved for Mucor spp. and Rhizopus spp.

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