[The establishment of method for identifying China vaccine strains and wild strains of measles virus]

Abstract

Objective: To establish a simple and quick method for identifying China vaccine strains and wild strains of Measles Virus.

Methods: To search the enzyme site in Hemagglutinin gene of measles virus for different domestic vaccine strains and wild strains of measles virus, and design the RT-PCR primer within the range covering the enzyme site, and then to confirm the specificity and sensibility of the RT-PCR method, and then identify the RT-PCR product by RFLP.

Results: The one-step RT-PCR method is sensitive, the measles virus of 4.64 TCID50 can be detected at least. No positive bands can be found in the non-measles virus strains, it means that the RT-PCR method has good specificity, the PCR products of Chian measles vaccine strains of Shang-191 and Chang-47 were all cut into two fragments (287 bp and 151 bp) by Afi II, but two measles wild virus strains can't be cut by Afl II.

Conclusion: The RT-PCR-RFLP method which we established is a rapid and simple method for identifying China vaccine strain and wild strain.