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. 2010 Mar;80(3):302-5.
doi: 10.1016/j.mimet.2010.01.003. Epub 2010 Jan 15.

Isolation and identification of arabinose mycolates of Cell Wall Skeleton (CWS) derived from Mycobacterium bovis BCG Tokyo 172 (SMP-105)

Yuko Uenishi  1 , Naoto Kusunose, Ikuya Yano, Makoto Sunagawa
Affiliations

Isolation and identification of arabinose mycolates of Cell Wall Skeleton (CWS) derived from Mycobacterium bovis BCG Tokyo 172 (SMP-105)

Yuko Uenishi et al. J Microbiol Methods. 2010 Mar.

Abstract

A unique hydrolysis method using a two-layer solution, consisting of diluted hydrochloric acid and toluene was developed to isolate whole arabinose mycolates from the cell wall skeleton of Mycobacterium bovis BCG Tokyo 172 (SMP-105) in order to reveal its pivotal role in enhancing immune responses against tumors.

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