Reinekea aestuarii sp. nov., isolated from tidal flat sediment

Abstract

Two Gram-negative, non-motile, non-pigmented and curved rod-shaped bacterial strains, designated IMCC4489(T) and IMCC4451, were isolated from a tidal flat sediment of the Yellow Sea. Strains IMCC4489(T) and IMCC4451 shared 99.9 % 16S rRNA gene sequence similarity and 78.5 % DNA-DNA relatedness, which suggested that they belonged to the same species. The isolates were most closely related to Reinekea blandensis MED297(T) (98.7-98.8 % 16S rRNA gene sequence similarity) and Reinekea marinisedimentorum DSM 15388(T) (95.3-95.4 %). DNA-DNA relatedness between the strains and R. blandensis CCUG 52066(T) was 31-34 %. Strains IMCC4489(T) and IMCC4451 could also be differentiated from the type strains of the two recognized Reinekea species by several phenotypic properties. The DNA G+C content was 51.3-51.5 mol% and the major isoprenoid quinone was Q-8. On the basis of the data obtained in this study, it is proposed that strains IMCC4489(T) and IMCC4451 represent a novel species, Reinekea aestuarii sp. nov. The type strain is IMCC4489(T) (=KCTC 22813(T) =KCCM 42938(T) =NBRC 106079(T)).