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. 2010 Feb;7(2):137-9.
doi: 10.1038/nmeth.1415. Epub 2010 Jan 17.

Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

Joachim Goedhart  1 , Laura van Weeren, Mark A Hink, Norbert O E Vischer, Kees Jalink, Theodorus W J Gadella Jr
Affiliations

Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

Joachim Goedhart et al. Nat Methods. 2010 Feb.

Abstract

Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (>3.7 ns) and high quantum yields (>0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor.

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