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. 2010 Mar;54(3):1343-6.
doi: 10.1128/AAC.01448-09. Epub 2010 Jan 19.

Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database

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Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database

Guangshun Wang et al. Antimicrob Agents Chemother. 2010 Mar.

Abstract

To identify novel anti-HIV-1 peptides based on the antimicrobial peptide database (APD; http://aps.unmc.edu/AP/main.php), we have screened 30 candidates and found 11 peptides with 50% effective concentrations (EC(50)) of <10 microM and therapeutic indices (TI) of up to 17. Furthermore, among the eight peptides (with identical amino acid compositions but different sequences) generated by shuffling the sequence of an aurein 1.2 analog, two had a TI twice that of the original sequence. Because antiviral peptides in the database have an arginine/lysine (R/K) ratio of >1, increases in the Arg contents of amphibian maximin H5 and dermaseptin S9 peptides and the database-derived GLK-19 peptide improved the TIs. These examples demonstrate that the APD is a rich resource and a useful tool for developing novel HIV-1-inhibitory peptides.

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Figures

FIG. 1.
FIG. 1.
Average Lys (K) and Arg (R) residue contents in groups of AMPs. Each of the peptide groups (antiviral, antifungal, anticancer, and antibacterial peptides and peptides toxic to mammalian cells) was obtained by searching the APD (http://aps.unmc.edu/AP/main.html). Groups: 1, 80 antiviral peptides; 2, 405 antifungal peptides; 3, 82 anticancer peptides; 4, 1,035 antibacterial peptides; 5, 157 peptides that are toxic to mammalian cells; and 6, all 1,405 peptides analyzed. The average amino acid contents in each group were obtained by initiating the statistical analysis program at the end of the peptide output. Detailed data for the original 525 peptides were reported in our database paper (20).

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