Antinociceptive and anti-inflammatory effects of ethanolic extracts of Glycine max (L.) Merr and Rhynchosia nulubilis seeds

Abstract

The aim of this study was to assess the in vivo potential of ethanolic extracts of Glycine max (L.) Merr. (SoRiTae) and Rhynchosia nulubilis (Yak-Kong) seeds as natural anti-nociceptive and anti-inflammatory agents. To assess the anti-nociceptive and anti-inflammatory potential, the ethanolic extracts of SoRiTae and Yak-Kong seeds were tested in arachidonic acid-induced ear edema, carrageenan induced paw edema, formalin-induced licking time, acetic acid induced writhing and hot plate-induced thermal stimulation in mice. The administration of ethanolic extracts of SoRiTae and Yak-Kong seeds evoked a significant effect of anti-nociceptive and anti-inflammatory activities as compared to standards aminopyrine and indomethacin. The ear edema, paw edema, paw licking time, pain and writhes in mice were significantly reduced (p < 0.05) as compared to the control. The results obtained in this study indicate that both SoRiTae and Yak-Kong soybeans possesses potential anti-nociceptive and anti-inflammatory activities.

Keywords: Glycine max; Rhynchosia nulubilis; anti-inflammatory activity; anti-nociceptive activity.

Figure 1.

Effects of SoRiTae and Yak-Kong ethanolic seed extracts on arachodonic acid-induced ear edema. Indo: Indomethacin (10 mg/kg); SL: SoRiTae (100 mg/kg); SH: SoRiTae (200 mg/kg), YL: Yak-Kong (100 mg/kg), YH: Yak-Kong (200 mg/kg). Each values was expressed as the mean ±S.E.M. (n = 6). ^a–c Means in the same column not sharing a common letter are significantly different (p < 0.05) by Duncan's multiple test.

Figure 2.

Effects of SoRiTae and Yak-Kong ethanolic seed extracts on carrageenan-induced paw edema. Indo: Indomethacin (10 mg/kg); SL: SoRiTae (100 mg/kg); SH: SoRiTae (200 mg/kg), YL: Yak-Kong (100 mg/kg), YH: Yak-Kong (200 mg/kg). Each values was expressed as the mean ±S.E.M. (n = 6). ^a–b Means in the same column not sharing a common letter are significantly different (p < 0.05) by Duncan's multiple test.

Figure 3.

Effects of SoRiTae and Yak-Kong ethanolic seed extracts on formalin-induced paw licking in mice. Amino: Aminopyrine (50 mg/kg); SL: SoRiTae (100 mg/kg); SH: SoRiTae (200 mg/kg), YL: Yak-Kong (100 mg/kg), YH: Yak-Kong (200 mg/kg). Each values was expressed as the mean ±S.E.M. (n = 6). ^a–d Means in the same column not sharing a common letter are significantly different (p < 0.05) by Duncan's multiple test.

Figure 4.

Effects of SoRiTae and Yak-Kong ethanolic seed extracts on acetic acid-induced writhing in mice. Amino: Aminopyrine (50 mg/kg); SL: SoRiTae (100 mg/kg); SH: SoRiTae (200 mg/kg), YL: Yak-Kong (100 mg/kg), YH: Yak-Kong (200 mg/kg). Each values was expressed as the mean ±S.E.M. (n = 6). ^a–c Means in the same column not sharing a common letter are significantly different (p < 0.05) by Duncan's multiple test.

Figure 5.

Effects of SoRiTae and Yak-Kong ethanolic seed extracts on thermally-induced pain in mice. Amino: Aminopyrine (50 mg/kg); SL: SoRiTae (100 mg/kg); SH: SoRiTae (200 mg/kg), YL: Yak-Kong (100 mg/kg), YH: Yak-Kong (200 mg/kg). Each values was expressed as the mean ±S.E.M. (n = 6). ^a–b Means in the same column not sharing a common letter are significantly different (p < 0.05) by Duncan's multiple test.