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. 1977 Oct;74(10):4248-50.
doi: 10.1073/pnas.74.10.4248.

Semisynthetic cytochrome c

L E Barstow, R S Young, E Yakali, J J Sharp, J C O'Brien, P W Berman, H A Harbury

Semisynthetic cytochrome c

L E Barstow et al. Proc Natl Acad Sci U S A. 1977 Oct.

Abstract

Horse heart cytochrome c can be split with cyanogen bromide into a heme peptide (residues 1-65) and a nonheme peptide (residues 66-104). In a process involving (i) complex formation between the two fragments and (ii) restoration of the severed peptide linkage, a fully active cytochrome c preparation can be re-formed. Use has been made of this process to couple the heme peptide to peptide 66-104 synthesized by the Merrifield solid-phase procedure. The semisynthetic product formed in this manner is indistinguishable from reconstituted cytochrome c prepared with nonsynthetic peptide 66-104.

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References

    1. J Biol Chem. 1960 Feb;235:531-4 - PubMed
    1. J Infect Dis. 1958 Nov-Dec;103(3):239-62 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3334-8 - PubMed
    1. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1400-6 - PubMed
    1. Adv Enzymol Relat Areas Mol Biol. 1969;32:221-96 - PubMed

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