McKay agar enables routine quantification of the 'Streptococcus milleri' group in cystic fibrosis patients
- PMID: 20093379
- DOI: 10.1099/jmm.0.016592-0
McKay agar enables routine quantification of the 'Streptococcus milleri' group in cystic fibrosis patients
Abstract
The 'Streptococcus milleri' group (SMG) has recently been recognized as a contributor to bronchopulmonary disease in cystic fibrosis (CF). Routine detection and quantification is limited by current CF microbiology protocols. McKay agar was developed previously for the semi-selective isolation of this group. Here, McKay agar was validated against a panel of clinical SMG isolates, which revealed improved SMG recovery compared with Columbia blood agar. The effectiveness of this medium was evaluated by appending it to the standard CF sputum microbiology protocols in a clinical laboratory for a 6-month period. All unique colony types were isolated and identified by 16S rRNA gene sequencing. Whilst a wide variety of organisms were isolated, members of the SMG were the most prevalent bacteria cultured, and McKay agar allowed routine quantification of the SMG from 10(3) to >10(8) c.f.u. ml(-1) directly from sputum. All members of the SMG were detected [Streptococcus anginosus (40.7 %), Streptococcus intermedius (34.3 %) and Streptococcus constellatus (25 %)] with an overall prevalence rate of 40.6 % in our adult CF population. Without exception, samples where SMG isolates were cultured at 10(7) c.f.u. ml(-1) or greater were associated with pulmonary exacerbations. This study demonstrates that McKay agar can be used routinely to quantify the SMG from complex clinical samples.
Similar articles
-
Development of real-time PCR assays for detection of the Streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16S rRNA genes.J Clin Microbiol. 2010 Apr;48(4):1150-60. doi: 10.1128/JCM.02082-09. Epub 2010 Feb 17. J Clin Microbiol. 2010. PMID: 20164275 Free PMC article.
-
The Streptococcus milleri group in chronic obstructive pulmonary disease.Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016 Sep;160(3):378-84. doi: 10.5507/bp.2016.017. Epub 2016 Apr 21. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016. PMID: 27132809
-
NAS agar is more suitable than McKay agar for primary culture of Streptococcus milleri group (SMG) fastidious bacteria, S. intermedius in particular.Folia Microbiol (Praha). 2017 Jan;62(1):11-15. doi: 10.1007/s12223-016-0470-y. Epub 2016 Sep 16. Folia Microbiol (Praha). 2017. PMID: 27638003
-
Molecular pathogenicity of Streptococcus anginosus.Mol Oral Microbiol. 2014 Aug;29(4):145-55. doi: 10.1111/omi.12056. Epub 2014 Jun 26. Mol Oral Microbiol. 2014. PMID: 24848553 Review.
-
The Streptococcus milleri group--an unrecognized cause of disease in cystic fibrosis: a case series and literature review.Pediatr Pulmonol. 2008 May;43(5):490-7. doi: 10.1002/ppul.20809. Pediatr Pulmonol. 2008. PMID: 18383109 Review.
Cited by
-
P. aeruginosa interactions with other microbes in biofilms during co-infection.AIMS Microbiol. 2023 Aug 10;9(4):612-646. doi: 10.3934/microbiol.2023032. eCollection 2023. AIMS Microbiol. 2023. PMID: 38173971 Free PMC article. Review.
-
Culture-enriched metagenomic sequencing enables in-depth profiling of the cystic fibrosis lung microbiota.Nat Microbiol. 2020 Feb;5(2):379-390. doi: 10.1038/s41564-019-0643-y. Epub 2020 Jan 20. Nat Microbiol. 2020. PMID: 31959969
-
Tobramycin-Treated Pseudomonas aeruginosa PA14 Enhances Streptococcus constellatus 7155 Biofilm Formation in a Cystic Fibrosis Model System.J Bacteriol. 2015 Oct 19;198(2):237-47. doi: 10.1128/JB.00705-15. Print 2016 Jan 15. J Bacteriol. 2015. PMID: 26483523 Free PMC article.
-
Cystic Fibrosis Airway Microbiome: Overturning the Old, Opening the Way for the New.J Bacteriol. 2018 Jan 24;200(4):e00561-17. doi: 10.1128/JB.00561-17. Print 2018 Feb 15. J Bacteriol. 2018. PMID: 29084859 Free PMC article. Review.
-
Pseudomonas aeruginosa Can Inhibit Growth of Streptococcal Species via Siderophore Production.J Bacteriol. 2019 Mar 26;201(8):e00014-19. doi: 10.1128/JB.00014-19. Print 2019 Apr 15. J Bacteriol. 2019. PMID: 30718303 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical