A review of current large-scale mouse knockout efforts
- PMID: 20095055
- DOI: 10.1002/dvg.20594
A review of current large-scale mouse knockout efforts
Abstract
After the successful completion of the human genome project (HGP), biological research in the postgenome era urgently needs an efficient approach for functional analysis of genes. Utilization of knockout mouse models has been powerful for elucidating the function of genes as well as finding new therapeutic interventions for human diseases. Gene trapping and gene targeting are two independent techniques for making knockout mice from embryonic stem (ES) cells. Gene trapping is high-throughput, random, and sequence-tagged while gene targeting enables the knockout of specific genes. It has been about 20 years since the first gene targeting and gene trapping mice were generated. In recent years, new tools have emerged for both gene targeting and gene trapping, and organizations have been formed to knock out genes in the mouse genome using either of the two methods. The knockout mouse project (KOMP) and the international gene trap consortium (IGTC) were initiated to create convenient resources for scientific research worldwide and knock out all the mouse genes. Organizers of KOMP regard it as important as the HGP. Gene targeting methods have changed from conventional gene targeting to high-throughput conditional gene targeting. The combined advantages of trapping and targeting elements are improving the gene trapping spectrum and gene targeting efficiency. As a newly-developed insertional mutation system, transposons have some advantages over retrovirus in trapping genes. Emergence of the international knockout mouse consortium (IKMP) is the beginning of a global collaboration to systematically knock out all the genes in the mouse genome for functional genomic research.
(c) 2010 Wiley-Liss, Inc.
Similar articles
-
Gene trapping in embryonic stem cells.Methods Enzymol. 2006;420:136-62. doi: 10.1016/S0076-6879(06)20008-9. Methods Enzymol. 2006. PMID: 17161697
-
Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.Nucleic Acids Res. 2005 Feb 1;33(2):e20. doi: 10.1093/nar/gni022. Nucleic Acids Res. 2005. PMID: 15687378 Free PMC article.
-
High throughput gene trapping and postinsertional modifications of gene trap alleles.Methods. 2011 Apr;53(4):347-55. doi: 10.1016/j.ymeth.2010.12.037. Epub 2011 Feb 19. Methods. 2011. PMID: 21334922
-
The gene trap resource: a treasure trove for hemopoiesis research.Exp Hematol. 2005 Aug;33(8):845-56. doi: 10.1016/j.exphem.2005.03.016. Exp Hematol. 2005. PMID: 16038776 Review.
-
Gene targeting in the mouse.Bioessays. 1994 Sep;16(9):633-8. doi: 10.1002/bies.950160907. Bioessays. 1994. PMID: 7980488 Review.
Cited by
-
All-in-one live: genes trapped, tagged and conditionally broken.Nat Methods. 2011 Jun;8(6):466-7. doi: 10.1038/nmeth.1617. Nat Methods. 2011. PMID: 21623352 No abstract available.
-
Using mouse models to study function of transcriptional factors in T cell development.Cell Regen. 2012 Oct 10;1(1):8. doi: 10.1186/2045-9769-1-8. eCollection 2012. Cell Regen. 2012. PMID: 25408871 Free PMC article. Review.
-
Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence.Sci Rep. 2020 Aug 24;10(1):14120. doi: 10.1038/s41598-020-70804-6. Sci Rep. 2020. PMID: 32839482 Free PMC article.
-
Successful derivation of EGFP-transgenic embryonic stem cell line from a genetically non-permissive FVB/N mouse.Am J Stem Cells. 2012 Jun 3;1(2):163-73. Print 2012. Am J Stem Cells. 2012. PMID: 23671805 Free PMC article.
-
Statistical Learning with Time Series Dependence: An Application to Scoring Sleep in Mice.J Am Stat Assoc. 2013 Jan 1;108(504):1147-1162. doi: 10.1080/01621459.2013.779838. J Am Stat Assoc. 2013. PMID: 24504359 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials