doi: 10.1021/la904375k.
Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide)
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- PMID: 20104867
- DOI: 10.1021/la904375k
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Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide)
Langmuir.
.
Abstract
We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. With this method, the conformation of long genomic DNA can be readily and reversibly switched between a very compact condensate and an unfolded macromolecule.
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