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. 2010 Apr;48(4):1425-7.
doi: 10.1128/JCM.02116-09. Epub 2010 Jan 27.

Differentiation of influenza B virus lineages Yamagata and Victoria by real-time PCR

Barbara Biere  1 , Bettina Bauer, Brunhilde Schweiger
Affiliations

Differentiation of influenza B virus lineages Yamagata and Victoria by real-time PCR

Barbara Biere et al. J Clin Microbiol. 2010 Apr.

Abstract

Since the 1970s, influenza B viruses have diverged into two antigenically distinct virus lineages called the Yamagata and Victoria lineages. We present the first real-time PCR assay for virus lineage differentiation to supplement classical antigenic analyses. The assay was successfully applied to 310 primary samples collected in Germany from 2007 to 2009.

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Figures

FIG. 1.
FIG. 1.
PCR assay validation. (A) Mean CT values (double reactions) of plasmid dilutions containing 107 to 102 genome equivalents of pYam and pVic were plotted against the cycle number. The slope and correlation (R2) are indicated. (B) Probit analyses were performed by examination of plasmid dilutions containing 100 to 0.1 genome equivalents of pYam and pVic in 10-fold reactions. Results were analyzed by using SPSS 17.0 statistics software.
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