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. 2010 Jan 18;15(1):385-98.
doi: 10.3390/molecules15010385.

Luteolin isolated from the flowers of Lonicera japonica suppresses inflammatory mediator release by blocking NF-kappaB and MAPKs activation pathways in HMC-1 cells

Ok-Hwa Kang  1 , Jang-Gi Choi, John-Hwa Lee, Dong-Yeul Kwon
Affiliations

Luteolin isolated from the flowers of Lonicera japonica suppresses inflammatory mediator release by blocking NF-kappaB and MAPKs activation pathways in HMC-1 cells

Ok-Hwa Kang et al. Molecules. .

Abstract

Luteolin (3',4',5,7-tetrahydroxylflavone) is a plant flavonoid and pharmacologically active agent that has been isolated from several plant species. In the present study, the effect of luteolin from the flowers of Lonicera japonica on phorbol 12-myristate 13-acetate (PMA) plus A23187-induced mast cell activation was examined. Luteolin significantly inhibited the induction of inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-8, IL-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) by PMA plus A23187. Moreover, luteolin attenuated cyclooxygenase (COX)-2 expression and intracellular Ca2+ levels. In activated HMC-1 cells, the phosphorylation of extra-signal response kinase (ERK 1/2) and c-jun N-terminal Kinase (JNK 1/2), but not p38 mitogen-activated protein kinase (p38 MAPK) were decreased by treatment of the cells with luteolin. Luteolin inhibited PMA plus A23187-induced nuclear factor (NF)-kappaB activation, IkappaB degradation, and luciferase activity. Furthermore, luteolin suppressed the expression of TNF-alpha, IL-8, IL-6, GM-CSF, and COX-2 through a decrease in the intracellular Ca2+ levels, and also showed a suppression of the ERK 1/2, JNK 1/2, and NF-kappaB activation. These results indicated that luteolin from the flowers of Lonicera japonica exerted a regulatory effect on mast cell-mediated inflammatory diseases, such as RA, allergy disease and IBD.

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Figures

Figure 1
Figure 1
Effect of luteolin on production of pro-inflammatory cytokines in PMA plus A23187-induced HMC-1 cells.
Figure 2
Figure 2
Effect of luteolin on gene expression of pro-inflammatory cytokines in PMA plus A23187-induced HMC-1 cells.
Figure 3
Figure 3
Effect of luteolin on COX-2 protein and mRNA expression in PMA plus A23187-induced HMC-1 cells.
Figure 4
Figure 4
Effect of luteolin on intracellular calcium levels.
Figure 5
Figure 5
Effect of luteolin on activation of MAPKs.
Figure 6
Figure 6
Effect of luteolin on the activation of NF-κB in HMC-1 cells.
See this image and copyright information in PMC

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