Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Mar;13(3):291-301.
doi: 10.1038/nn.2486. Epub 2010 Jan 31.

An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons

Catia Andreassi  1 , Carola Zimmermann, Richard Mitter, Salvatore Fusco, Serena De Vita, Adolfo Saiardi, Antonella Riccio
Affiliations

An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons

Catia Andreassi et al. Nat Neurosci. 2010 Mar.

Erratum in

  • Nat Neurosci. 2010 Aug;13(8):1033. Devita, Serena [corrected to De Vita, Serena]

Abstract

mRNA localization is an evolutionary conserved mechanism that underlies the establishment of cellular polarity and specialized cell functions. To identify mRNAs localized in subcellular compartments of developing neurons, we took an original approach that combines compartmentalized cultures of rat sympathetic neurons and sequential analysis of gene expression (SAGE). Unexpectedly, the most abundant transcript in axons was mRNA for myo-inositol monophosphatase-1 (Impa1), a key enzyme that regulates the inositol cycle and the main target of lithium in neurons. A novel localization element within the 3' untranslated region of Impa1 mRNA specifically targeted Impa1 transcript to sympathetic neuron axons and regulated local IMPA1 translation in response to nerve growth factor (NGF). Selective silencing of IMPA1 synthesis in axons decreased nuclear CREB activation and induced axonal degeneration. These results provide insights into mRNA transport in axons and reveal a new NGF-responsive localization element that directs the targeting and local translation of an axonal transcript.

PubMed Disclaimer

Comment in

  • The longer U(T)R, the further you go.
    Cosker KE, Segal RA. Cosker KE, et al. Nat Neurosci. 2010 Mar;13(3):273-5. doi: 10.1038/nn0310-273. Nat Neurosci. 2010. PMID: 20177416 No abstract available.

References

    1. Nat Protoc. 2007;2(10):2520-8 - PubMed
    1. Annu Rev Physiol. 2003;65:761-89 - PubMed
    1. Nat Rev Mol Cell Biol. 2001 May;2(5):327-38 - PubMed
    1. J Cell Biol. 2007 Sep 10;178(6):965-80 - PubMed
    1. Front Biosci. 2008 Jan 01;13:856-66 - PubMed

Publication types

MeSH terms

Substances