Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb 1;66(Pt 2):201-3.
doi: 10.1107/S1744309109053688. Epub 2010 Jan 28.

Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana

Zhu-Bing Shi  1 , Hong-Hua Ge, Ping Zhao, Min Zhang
Affiliations

Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana

Zhu-Bing Shi et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

VSP1 is a defence protein in Arabidopsis thaliana that may also be involved in control of plant development. The recombinant protein has been overexpressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method. The crystal diffracted to 1.9 A resolution and a complete X-ray data set was collected at 100 K using Cu Kalpha radiation from a rotating-anode X-ray source. The crystals belonged to space group C2. As there are no related structures that could be used as a search model for molecular replacement, work is in progress on experimental phasing using heavy-atom derivatives and selenomethionine derivatives.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Crystals of recombinant VSP1 grown by the sitting-drop method. The crystals grew to dimensions of ∼0.02 × 0.1 × 0.15 mm.
See this image and copyright information in PMC

References

    1. Allen, K. N. & Dunaway-Mariano, D. (2004). Trends Biochem. Sci.29, 495–503. - PubMed
    1. Berger, S., Bell, E., Sadka, A. & Mullet, J. E. (1995). Plant Mol. Biol.27, 933–942. - PubMed
    1. Berger, S., Mitchell-Olds, T. & Stotz, H. U. (2002). Physiol. Plant.114, 85–91. - PubMed
    1. Bradford, M. M. (1976). Anal. Biochem.72, 248–254. - PubMed
    1. Burroughs, A. M., Allen, K. N., Dunaway-Mariano, D. & Aravind, L. (2006). J. Mol. Biol.361, 1003–1034. - PubMed

Publication types

MeSH terms